Abstract: To construct Shcbp1 lentiviral interference vector to explore the biological function of SHCBP1 in melanoma B16 cells, first,the shRNA-1 and shRNA-2 that target to interfere with the mouse Shcbp1 gene and the control sequence shRNA-NC were designed and connected to the lentiviral backbone vector, HEK293 cells were used to package the lentiviral particles to infect B16 cells, the interference efficiency was tested and CCK-8 and flow cytometry were used to detect cell viability and cell cycle. Western Blot was used to detect the expression of proliferation-related genes. The results showed that knockdown of Shcbp1 significantly inhibited the proliferation of B16 cells; flow cytometry results showed that knockdown of Shcbp1 blocked B16 cells in the G1 phase; the phosphorylation level of ERK1/2 decreased and the expression level of p21 increased. These results indicate that SHCBP1 may inhibit the transcription of P21 through the ERK1/2 signaling pathway and accelerate the progress of B16 cells from G1 to S phase

Key words: Shcbp1, cell proliferation, B16 cell, lentiviral interference